Cilj ovog istraživanja je bio ispitati uticaj primjene razlicitih koncentracija sintetski proizvedenih biljnih hormonana parametre razvoja presadnica paprike ( Capsicum annuum L. cv. 'Sivrija'). Biljni hormoni koristeni u ovom ogledu bili su indol-sircetna kiselina (IAA), indol-butilna kiselina (IBA) i benzil adenin (BA), a svaki od njih primijenjen je u pet razlicitih koncentracija: 100, 300, 500, 1000 i 3000 mg l -1 .Od parametara kvaliteta presadnica paprike u ovom istraživanju ispitivani su: masa svježe tvari korijena, broj korijenovih dlacica po jedinici povrsine korijena, povrsina listova, te sadržaj fotosintetskih pigmenata u listovima.Najveci uticaj na povecanje ispitivanih parametara kvaliteta presadnica paprike je iskazala primjenahormonaIAA i IBA u koncentracijama 100 i 300 mg l -1 , te se iste preporucuju za primjenu kod uzgoja presadnica paprike ( Capsicum annuum L. cv. 'Sivrija').U varijantama gdje su hormoni IAA, IBA i BA primijenjeniu koncentracijama od 500 i vise mg l -1 mlade presadnice paprike nisu uspjele nastaviti svoj životni ciklus, te je doslo do njihovog venuca, sto se može objasniti cinjenicom da previsoka koncentracija hormona može iskazati i herbicidni ucinak na biljku, posebno u ranim stadijima razvoja biljke.

AbstractA complete characterization of the different physico-chemical properties of nanoparticles (NPs) is necessary for the evaluation of their impact on health and environment. Among these properties, the surface characterization of the nanomaterial is the least developed and in many cases limited to the measurement of surface composition and zetapotential. The biological surface adsorption index approach (BSAI) for characterization of surface adsorption properties of NPs has recently been introduced (Xia et al. Nat Nanotechnol 5:671–675, 2010; Xia et al. ACS Nano 5(11):9074–9081, 2011). The BSAI approach offers in principle the possibility to characterize the different interaction forces exerted between a NP's surface and an organic—and by extension biological—entity. The present work further develops the BSAI approach and optimizes a solid-phase microextraction gas chromatography–mass spectrometry (SPME/GC-MS) method which, as an outcome, gives a better-defined quantification of the adsorption properties on NPs. We investigated the various aspects of the SPME/GC-MS method, including kinetics of adsorption of probe compounds on SPME fiber, kinetic of adsorption of probe compounds on NP's surface, and optimization of NP's concentration. The optimized conditions were then tested on 33 probe compounds and on Au NPs (15 nm) and SiO2 NPs (50 nm). The procedure allowed the identification of three compounds adsorbed by silica NPs and nine compounds by Au NPs, with equilibrium times which varied between 30 min and 12 h. Adsorption coefficients of 4.66 ± 0.23 and 4.44 ± 0.26 were calculated for 1-methylnaphtalene and biphenyl, compared to literature values of 4.89 and 5.18, respectively. The results demonstrated that the detailed optimization of the SPME/GC-MS method under various conditions is a critical factor and a prerequisite to the application of the BSAI approach as a tool to characterize surface adsorption properties of NPs and therefore to draw any further conclusions on their potential impact on health. Graphical AbstractThe basic principle of SPME/GC-MS method for characterization of nanoparticles surface adsorption forces