Synthesis, structure and biological activity of 3-substituted derivatives of 4-hydroxycoumarin
Background and Purpose: Due to the exceptional reactivity of 4-hydroxycoumarin, as well as the versatile biological activity of coumarin derivatives, the synthesis of 3-substituted derivatives of 4-hydoxycoumarin were carried out, and antibacterial and antifungal activity were tested. We assumed that newly-prepared derivatives might have antifungal and antibacterial activity. Material and Methods: Microbiological activity of compounds was tested by the diffusion method on the species of bacteria Escherihia coli 113-1, Pseudomonas aeruginosa ATCC 9027, Staphylococcus aureus SG 511, and fungi Candida albicans ATCC 10231. For comparison of results, the antibacterial activity of currently used antibiotics to the same species ofbacteria was tested. Results: The synthesis of derivatives of 3-cinammoyl-4-hydroxy-coumarin and azomethines of 4-hydroxycoumarin was carried out. Both groups of compounds showed significant microbiological activity. Conclusion: All synthesized compounds showed antimycotic and antibacterial activity. Derivatives of3-cinnamoyl-4-hydroxycoumarin in relation to azomethines of 4-hydroxycoumarin showed better activity in case of all bacteria and fungi species. The best antibacterial activity was shown by the species which had -Cl or -OCH 3 as substituents, and the best antimycotic activity was shown by the species which contained Cl.