P-02 . 03 . 3-007 Fine tuning of selective toxicity of anti-tumorigenic compounds

s submitted to the 41st FEBS Congress, which was planned for Kuşadası, Turkey from 3rd to 8th September 2016, and accepted by the Congress Organizing Committee are published in this Special Issue of The FEBS Journal. Unfortunately, the Congress was cancelled by FEBS after the excellent scientific programme was compromised by an insufficient number of confirmed speakers, and so the authors of these abstracts were not able to present their work at the event*. Late-breaking abstracts and abstracts withdrawn after Congress cancellation are not included in this issue. About these abstracts Abstracts submitted to the Congress are not peer-reviewed. In addition, abstracts are published as submitted and are not copyedited prior to publication. We are unable to make corrections of any kind to the abstracts once they are published. Indexing Abstracts published in The FEBS Journal Special Issue for the 41st FEBS Congress will be included individually in the Conference Proceedings Citation Index published by Web of Science. How to cite these abstracts AuthorOne, A., AuthorTwo, B. (2016). Abstract title. FEBS J, 283: Abstract number**. doi:10.1111/febs.13903 * An optional closed online presentation opportunity of short duration on the Congress website was offered after Congress cancellation and may be taken up by some abstract authors. ** Each abstract has been given a unique number beginning with either the letters P or ST; the next part relates to the session in which the speed talk or poster will be presented. 01.02 RNA biology, biogenesis and processing 02.03 Autophagy: Regulation mechanisms 02.04 Mechanisms and regulation of protein translocation 02.05 Intracellular organization 02.06 Human microbiome (microbiota) 02.07 Extracellular matrix and metalloproteinases 09.04 Chemical and biochemical aspects of oxidative stress Mis Miscellaneous 428 The FEBS Journal 283 (Suppl. 1) (2016) 428 DOI: 10.1111/febs.13903 © 2016 The Authors. The FEBS Journal © 2016 FEBS This addendum corrects errors in the Speed Talks and Poster sessions of the Supplement S1. The following abstracts were omitted by mistake from the original line-up. In addition, the abstracts P-02.03.3-002 and P-09.04.4-104 were truncated in the original document and are reproduced in full here. FEBS_v283_s1_13903.indd 1 10/12/2016 2:00:46 PM OMITTED POSTER SESSIONS Tuesday 6 September 12:30–14:30 Autophagy: Regulation mechanisms P-02.03.3-002 Apoptotic and necrotic effects of low dose bisphenol A in SHSY5Y neuroblastoma cells B. Ayazg€ok, T. T€ uyl€ u K€ uc € ukkilinc Hacettepe University Faculty of Pharmacy, Ankara, Turkey Bisphenol A (BPA) is a commonly used chemical in industry to make plastics. “Low-dose” term has been expressed for the first time in studies with BPA in 2001. The value of low dose was received as <1 lM for BPA in in vitro studies. Nowadays majority of the population as a result of today’s lifestyle exposured to low doses BPA chronically, thus importance of low-dose toxicity studies is revealed. In this study we aimed to examine cytotoxicity composed by low dose BPA in SHSY5Y cells in terms of appoptotic and necrotic effects.SHSY5Y cells was seeded at 300.000 cells per well in 6-well plates and cultured in DMEM at 37°C with 5% CO2.SH-SY5Y cells were treated with low dose(1 pM, 1 nM) of BPA. Plate was incubated for 24 and 48 hours. After the incubation period, samples were pooled then washed with PBS in two times and 100 ll aliquot of cells from each sample was centrifuged at 12000 g, +4 °C for 2 minutes and resuspended in 100 ll annexin binding buffer added to 5 ll Annexin V and incubated at room temperature in the dark for 20 minutes. Then, samples were centrifuged and resuspended again in 100 ll of the same buffer and added with 1 ll PI at room temperature for 1–4 minutes and analysed at Tali Image-Based Cytometer. Reducing cell viability of low dose BPA in SHSY5Y neuroblastoma cells is revealed by MTT by our group in our previous studies. Cytotoxicity studies are conducted for 1 pm and 1 nm of BPA in 48 hours by taking into account this result. It is obtained that 1pM reduced cell viability to % 63 and 1 nM BPA decreased cell viability to %78. It is clearly ocuured via examinate of necrotic effects that group treated with 1 pM and 1 nM BPA was significiantly different from control group. It has observed group treated with 1 pM and 1 nM BPA has importantly difference compared control group. The findings obtained from this study explain the cytotoxicity of BPA in SHSY5Y cells through necrotic and late apoptotic pathways. P-02.03.3-005 Lack of Atg5 expression diminished apoptotic potential of cdk inhibitors due to increased Bcl-2 expression in MEF cells A. E. Nezir, E. D. Arisan, A. Coker-Gurkan, P. Obakan,