Preliminary definition of analgesic effect of newly synthesized derivatives of pyrazolin and quinoline carbonic acids

Background and Purpose: On the basis of structural similarity of newly synthesized compounds and non-steroidal anti-inflammatory drugs, we assumed that these compounds might have analgesic activity. Materials and Methods:Analgesic effects of newly synthesized compounds were analyzed on the albino mice of both genders. The sense of pain was caused by thermal stimulus by the method of hot plate. Individual analgesic effect of newly synthesized compounds was measured 30, 60, 90 and 120 minutes after a single oral administration in a dose of 35 mg/kg. Physiological solution in the same volume was administered to a control group. The surveyed compounds have the following chemical names: A = 1-phenyl-5-(4-acetamido-phenyl)-3-(4-hydroxycoumarin)-Δ 2 -pyrazolin, B = 1-phenyl-5-(3-chlorphenyl)-3-(4-hydroxycoumarin)-Δ 2 -pyrazolin, C = /1/-benzopyrano-(4,3b)-7-methyl-quinoline-6-on, D = 2-(2-hydroxyphenyl)-4-methylqui-noline-3-carbonic acid. Results: Latency period for the compound A was significantly extended (p<0.001) 30 and 60 minutes after application as compared to the control group. However, 90 and 120 minutes after the application, this difference was not statistically significant (p=0,806 and p =0, 773) (t-test). The latency period for the compounds B and C as compared to the control group was significantly extended (p<0.001) in all observed time intervals. Similarly, the latency period for the compound D was extended 30 and 60 minutes after the application and was significant as compared to the control group (p<0.001). Latency was also increased when registered after 90 and 120 minutes (p=0.008 and p=0.026). Conclusions: After oral application, the investigated compounds showed analgesic effects to the sense of pain caused by a thermal stimulus.