Antimicrobial Effect of the Rhizoma and Root Extract Fractions Potentilla Speciosa Willd. And Potentilla Tommasiniana F. Schultz.,Rosaceae
Continuing our previous researches of antimicrobial activity of plant sorts P. speciosa and P. tommasiniana, which are characteristic for Balkan Peninsula, we examined ethyl acetate, ethanol and acetone rhizoma and root extracts (concentration 1:1) of the plants examined. To examine the antibiotic effects we used diffusion method from Ph. Eur ed. 4 as the method for antibiotics examinations. In this work the activity of prepared extracts was examined on Bacillus subtilis ATCC 6632, Staphylococcus aureus ATCC 6538, Staphylococcus epidennidis ATCC 2228 and Escherichia coli ATCC 8739(2). The base used for Bacillus subtilis ATCC 6632 had the following composition: peptone 5 g, meat extract 2,4 g, agar 15 g and purified water up to 1000 g. Base A for antibiotics examination by diffusion method was used to examine Staphylococcus aureus ATCC6538, Staphylococcus epidennidis ATCC 2228 and Escherichia coli ATCC 8739. Ethyl acetate extracts were also examined on Silicagel G60 plates by thin layer chromatography with mobile phase: glacial acetic acid, ether, hexane, ethyl acetate. (20:20:20:40 V/V/V/V). The examined extracts showed antimicrobial effect, and obtained chromatographs contribute to better knowledge of the analytics of examined plant material.