Somatic embryogenesis and in vitro plantlet regeneration of Lilium martagon L. var. cattaniae Vis.
Karalija, E., Trbojevic, S., Paric, A.: Somatic embryogenesis and in vitro plantlet regeneration of Lilium martagon L. var. cattaniae Vis.. Biologica Nyssana, 1 (1-2), December 2010: 57-60. In this study organogenic capacity of two different explants type (leaves and whole bulbs) of Lilium martagon L. var. cattaniae Vis. was examined. For induction of in vitro somatic embryogenesis and adventitive regeneration different concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzilaminopurine (from 0,25 mg/l to 8,00 mg/l) added to MS basal medium were used. Our results indicate that concentration of 0,5 mg/l 2,4-dichlorophenoxyacetic acid and 4 mg/l 6- benzilaminopurine promoted somatic embryogenesis from leaves of Lilium martagon var. cattaniae, while all other concentrations promoted direct shoot regeneration from bulb explants. Root formation was induced on MS basal medium with 0,2 mg/l indole butyric acid. These plantlets were acclimatized well in a greenhouse conditions.