Background and purpose: Ants are a dominant terrestrial insect group and rank among the most abundant and diverse animals on land, playing significant ecological and economic roles. Due to their global diversity and the high morphological similarity among closely related species, accurate identification of ants can be difficult. The aim of this study was to assess the species status within one of the most important ant groups – Lasius genus, subgenus Lasius s. str. – in Bosnia and Herzegovina, using a combination of morphological and molecular approaches. Materials and methods: Forty-two individuals were collected from four localities and identified using appropriate morphological keys. DNA barcoding was performed to complement morphological identification, and sequences were deposited in GenBank. A Maximum Likelihood phylogenetic tree was constructed using MEGA11, incorporating both the sequences from this study and selected sequences from the BOLD database. Results: We identified three species - Lasius niger, L. platythorax and L. emarginatus, by applying both approaches. Specific dark-brown mesosoma and a new haplotype were recorded for L. emarginatus. Conclusions: The observed morphometric differences and a discovery of a new Lasius emarginatus haplotype suggest that the diversity of species within the subgenus in Bosnia and Herzegovina is greater than previously recognized. Further research is warranted to explore the regional diversity and ecological roles of Lasius species.

Biological invasions are a major driver of biodiversity loss, yet inconspicuous or “cryptic” species often escape detection and public awareness, limiting management responses. We investigated the freshwater jellyfish Craspedacusta sowerbii , likely native to China and now present on six continents, through a 22‐month multilingual online survey across 17 European countries ( n  = 1388), to assess public knowledge, perceptions and views on its management. Results revealed low public awareness: over 80% of respondents did not know the scientific name, 49% misidentified its freshwater habitat as marine and only 33% recognized it as non‐native. Aesthetic perceptions were largely positive, but mass occurrences triggered caution and direct contact was rare and usually harmless. Bayesian Network analysis showed that direct observation—rather than demographic factors or scientific knowledge—was the strongest predictor of concern and support for public discussion. While C. sowerbii was not widely perceived as a local threat, many respondents acknowledged its potential ecological implications and supported greater public awareness and management actions. Our findings highlight how visual subtlety and limited media coverage reduce detection and prioritization of cryptic invasive alien species (IAS), hindering early warning systems and policy development. Leveraging citizen science and experiential engagement can improve recognition and reporting, complement formal monitoring and support more inclusive management strategies. We recommend integrating cryptic invasions like that of C. sowerbii into IAS frameworks and policies, emphasizing public participation and awareness to address overlooked, but potentially impactful species. Read the free Plain Language Summary for this article on the Journal blog.

This study provides useful insight into the current state and recent developments in blue (marine) biotechnology education (BBE) in Europe. A questionnaire assessing awareness and interest in blue biotechnology (BB) was conducted among students in their final year of upper secondary school in a selection of European countries. Results demonstrate that there are great regional differences in students’ recognition of BB; recognition of BB is relatively poor (though biotechnology knowledge is relatively good). This illustrates the lack of early education and ocean literacy. The interest in BB studies varied by country. Non-formal science, technology, engineering, and mathematics (STEM) activities, especially visits to research institutes and private businesses, help raise awareness. The review of education programs conducted in this study revealed that only a small number of programs are dedicated to BB and that it is not well integrated into existing curricula. Early ocean literacy, lifelong learning opportunities, and the inclusion of entrepreneurship and interdisciplinary skills in education are essential for developing a workforce capable of driving the future of the blue bioeconomy within the broader objectives of the EU Blue Economy Strategy and European Union (EU) Bioeconomy Strategy. To advance BBE, we propose the following recommendations: 1) incorporate and increase ocean literacy and BBE at the primary and secondary school levels; 2) support lifelong learning and adaptation to emerging technologies; 3) build collaborative ocean education networks; 4) bridge education communities, research and industry; 5) encourage entrepreneurship; 6) harmonise postgraduate BB programs; 7) establish joint European MSc and PhD degrees; and 8) align funding and BBE activities with national and regional gross domestic product (GDP) contributions for the BB sector.

In terms of ichthyology, Bosnia and Herzegovina (BiH) is one of the most interesting parts of Southeast Europe, due to its rich biodiversity and high level of endemism. Despite its relevance, the entire territory has been poorly explored. Here, we provide an updated inventory of the current state of knowledge on fishes, including lampreys, from the freshwaters of BiH by hydrographic basin, with recent distributional data and updated taxonomic status reviewed and compared with previous lists. The checklist was compiled based on the existing scientific and grey literature, technical reports, scientific congresses, academic dissertations, and unpublished/personal observations. In total, 123 species including diadromous and euryhaline fishes have been documented in BiH freshwaters to date. Of these, 110 are primarily freshwater. In comparison to the last published monography (Sofradžija 2009), we present a 9% increase in species number (11 species), resulting mainly from taxonomic re-evaluations of existing taxa on the basis of new information and the adoption of a new changes in the taxonomic status of several species. Among the valid primarily freshwater species, 87 are native and 23 are non-native. A total of 38 endemic species have restricted distribution, and are threatened by numerous anthropogenic pressures. Four species are considered endemic only to BiH: Cobitis herzegoviniensis Buj & Šanda, 2014; Phoxinellus pseudalepidotus Bogutskaya & Zupančič, 2003; Telestes dabar Bogutskaya, Zupančič, Bogut & Naseka, 2012; and T. metohiensis (Steindachner, 1901). In total, 75 genera and 34 families are represented: Leuciscidae is represented by 37 species, the Salmonidae by 13, followed by the Cyprinidae, Cobitidae and Percidae, each with eight species. The native species richness follows a pattern similar to that observed in other southern European countries. A national list of endangered species has not yet been proposed to BiH and management strategies for their protection or conservation are also not implemented. Hopefully, this updated checklist will serve as a basis for future research aimed at understanding the origin and status of conservation of the BiH fishes diversity, and supporting effective management and conservation programmes.

The Balkan mountain ranges are major hotspots of genetic diversity and endemism, yet many species remain poorly studied. One such species is Alyssum bosniacum, a narrow endemic of the Central Dinaric Alps. To fill this gap, we examined 143 individuals from 15 populations across the species’ range using flow-cytometric ploidy determination, amplified fragment length polymorphisms (AFLPs), nuclear microsatellites, and chloroplast DNA sequences. Microsatellite data revealed two genetic clusters, showing moderate differentiation and relatively high diversity. AFLP profiles indicated shallow but geographically structured variation, while chloroplast haplotypes showed limited divergence and regional clustering. Our data suggest possible persistence in multiple microrefugia within the Central Dinaric Alps, although further evidence is needed to confirm this scenario. Despite range fragmentation, genetic variation within the population remains high, indicating evolutionary resilience and supporting the species’ long-term future population stability under current conditions.

RRI, ethical standards, the Nagoya and Cartagena Protocols, and IP frameworks influence natural products R&D&I and the bioeconomy, providing a foundation for sustainable and equitable resource use while promoting innovation and growth.

Background and purpose: Three genera and eleven species of Glossosomatidae family have been confirmed in Bosnia and Herzegovina. However, there is limited research on the biodiversity of this family in the region, especially on molecular data. With this paper, we contribute to the knowledge on the Glossosomatidae family with the first molecular records for two species for Bosnia and Herzegovina. Materials and methods: Larval samples of caddisflies were collected in April 2019 and 2021 at the area of the Natural Monument the Spring of Bosna River followed by morphological identification. Molecular confirmation of species was done using DNA barcoding, targeting the cytochrome c oxidase subunit I (COI) region using Folmer’s primers and degenerated primers LCO1490-JJ and HCO2198-JJ. All obtained PCR products were bidirectionally sequenced using the same primers as in PCR. Results: Out of 52 specimens, 18 were morphologically determined to species level. A DNA barcode was successfully generated for 16 specimens. Eight sequences were generated for Synagapetus krawanyi, revealing three haplotypes previously absent from the BOLD database. Four sequences were obtained for S. iridipennis, with one new haplotype for BOLD database. Conclusions: We present new data on species distribution and genetic diversity of S. krawanyi and S. iridipennis from Bosnia and Herzegovina. This study contributes to the knowledge of caddisfly biodiversity and the Global Trichoptera Barcode Initiative by adding new records from two underrepresented species in the BOLD database.

Polyploidy is a powerful mechanism driving genetic, physiological, and phenotypic changes among cytotypes of the same species across both large and small geographic scales. These changes can significantly shape population structure and increase the evolutionary and adaptation potential of cytotypes. Alyssum moellendorfianum, an edaphic steno-endemic species with a narrow distribution in the Balkan Peninsula, serves as an intriguing case study. We conducted a comprehensive analysis of genetic diversity and population structure across the species’ range, employing an array of genetic techniques (nuclear microsatellites, amplified fragment length polymorphisms, and plastid DNA sequences), flow cytometry (FCM), morphometry, and pollen analysis. The study reveals two genetic lineages: spatially distributed diploid and tetraploid cytotypes. Clear divergence between diploids and tetraploids was shown by AFLP, while plastid DNA sequences confirmed private haplotypes in each of the studied populations. Higher genetic diversity and allelic richness following the north-south pattern were documented in tetraploids compared to diploids, as indicated by nuclear microsatellites. Morphometric analysis via principal component analysis (PCA) and canonical discriminant analysis (CDA) did not reveal any divergence between diploid and tetraploid cytotypes. Nonetheless, a distinction in pollen size was clearly observed. The results suggest an autopolyploid origin of tetraploids from diploid ancestors. Despite the population fragmentation in a very small geographic range, these populations harbour high genetic diversity, which would allow them to remain stable if natural processes remain undisturbed.

The Kunming-Montreal Global Biodiversity Framework (GBF) is the most ambitious agreement on biodiversity conservation and sustainable use to date. It calls for a whole-of government and whole-of-society approach to halt and reverse biodiversity loss worldwide. The Monitoring Framework of the GBF lays out how Parties to the Convention on Biological Diversity (CBD) are expected to report their progress. A CBD expert group provided guidance on its implementation, including a gap analysis to identify the strengths and limitations of the indicators in the Monitoring Framework. We present the results of the gap analysis, highlight where more work is needed and provide recommendations on implementing and improving monitoring to allow effective and comprehensive tracking of the GBF’s ambition. We find that with the headline and binary indicators, which Parties are required to use, the Monitoring Framework fully covers 19% of the GBF’s ambition and partially covers an additional 40%. Including disaggregations of the headline indicators improves coverage to 22% fully and an additional 41% partially. Adding optional (component and complementary) indicators brings full coverage to 29% with an additional 47% partial coverage. No indicators are available for 12% of the GBF. In practice, the coverage of the Monitoring Framework will depend on which indicators (headline and binary as well as component and complementary) and disaggregations are used by countries. Disaggregations are particularly relevant to monitor the cross-cutting considerations defined under section C. Substantial investment is required to collect the necessary data to compute indicators, infer change, and effectively monitor progress. We highlight important next steps to progressively improve the efficacy of the Monitoring Framework.

The Kunming-Montreal Global Biodiversity Framework (GBF) is the most ambitious agreement on biodiversity conservation and sustainable use to date. It calls for a whole-of government and whole-of-society approach to halt and reverse biodiversity loss worldwide. The Monitoring Framework of the GBF lays out how Parties to the Convention on Biological Diversity (CBD) are expected to report their progress. A CBD expert group provided guidance on its implementation, including a gap analysis to identify the strengths and limitations of the indicators in the Monitoring Framework. We present the results of the gap analysis, highlight where more work is needed and provide recommendations on implementing and improving monitoring to allow effective and comprehensive tracking of the GBF’s ambition. We find that with the headline and binary indicators, which Parties are required to use, the Monitoring Framework fully covers 19% of the GBF’s ambition and partially covers an additional 40%. Including disaggregations of the headline indicators improves coverage to 22% fully and an additional 41% partially. Adding optional (component and complementary) indicators brings full coverage to 29% with an additional 47% partial coverage. No indicators are available for 12% of the GBF. In practice, the coverage of the Monitoring Framework will depend on which indicators (headline and binary as well as component and complementary) and disaggregations are used by countries. Disaggregations are particularly relevant to monitor the cross-cutting considerations defined under section C. Substantial investment is required to collect the necessary data to compute indicators, infer change, and effectively monitor progress. We highlight important next steps to progressively improve the efficacy of the Monitoring Framework.