Digestate, a key byproduct of anaerobic digestion (AD), holds residual methane potential (RMP) that must be stabilized or recovered to prevent greenhouse gas emissions after field use. Thermal hydrolysis (TH), typically a pretreatment for AD, improves biogas production. This study assesses RMP in digestates from food waste (FW) and sewage sludge (SS) biogas plants, treated with TH at 160 and 190 °C. For the liquid fraction, FW digestate at 160 °C yielded 1.5 times more methane than untreated digestate, while SS digestate showed a threefold increase. The solid fraction of FW digestate at 160 °C had 1.4 times higher methane yield than untreated, but SS digestate produced less methane after TH. Adding sulfuric acid after TH increased phosphate release but reduced methane production in both digestates. Overall, TH as a post-treatment enhanced organic content release into the liquid fraction, enhancing methane yield, while acid addition improved phosphorus solubility, thereby enhancing digestate's nutrient value.

Tracer testing in reservoir formations is utilised to determine residual oil saturation as part of optimum hydrocarbon production. Here, we present a novel detection method of liquid organic compounds by monodisperse SiO2 nanoparticles (NPs) containing two luminophores, a EuIII:EDTA complex and a newly synthesised fluorophore based on the organic boron-dipyrromethene (BODIPY)-moiety. The particles exhibited stable EuIII PL emission intensity with a long lifetime in aqueous dispersion. The fluorescence of the BODIPY was also preserved in the aqueous environment. The ratiometric PL detection technique was demonstrated by using toluene and 1-octanol as model compounds of crude oil. The optimal synthesis conditions were found to give NPs with a diameter of ~ 100 nm, which is suitable for transport through porous oil reservoir structures. The cytotoxicity of the NPs was confirmed to be very low for human lung cell and fish cell lines. These findings demonstrate the potential of the NPs to replace the hazardous chemicals used to estimate the residual oil saturation. Moreover, the ratiometric PL detection technique is anticipated to be of benefit in other fields, such as biotechnology, medical diagnostics, and environmental monitoring, where a reliable and safe detection of a liquid organic phase is needed.

Intrinsically fluorescent carbon dots may form the basis for a safer and more accurate sensor technology for digital counting in bioanalytical assays. This work presents a simple and inexpensive synthesis method for producing fluorescent carbon dots embedded in hollow silica particles. Hydrothermal treatment at low temperature (160 °C) of microporous silica particles in presence of urea and citric acid results in fluorescent, microporous and hollow nanocomposites with a surface area of 12 m2 /g. High absolute zeta potential (-44 mV) at neutral pH demonstrates the high electrosteric stability of the nanocomposites in aqueous solution. Their fluorescence emission at 445 nm is remarkably stable in aqueous dispersion under a wide pH range (3-12) and in the dried state. The biocompatibility of the composite particles is excellent, as the particles were found to show low genotoxicity at exposures up to 10 μg/cm2 .