Aims: Cardiovascular alterations contribute to a high mortality rate in patients with end-stage renal disease (ESRD). The aims of the present study are to evaluate left ventricular (LV) function and common carotid artery (CCA) parameters and to determine risk factors associated with these changes in patients undergoing peritoneal dialysis (PD). Methods: This longitudinal prospective study was conducted in 50 ESRD patients in whom PD had been initiated and who were observed for 18 months after the commencement of dialysis treatment, with echocardiography and CCA ultrasound parameter evaluation. Results: LV hypertrophy was observed in 78% of patients at baseline and in 60% after 18 months of PD treatment. LV systolic and diastolic function was found to be significantly better after 18 months of PD treatment. Examining predictors of LV systolic function, it was found that total cholesterol was an independent positive predictor and endothelin-1 (ET-1) an independent negative predictor of LV systolic function after 18 months of treatment with PD (p < 0.001). Independent negative predictors of diastolic LV function were hemoglobin and type 2 diabetes mellitus, and daily collection of urine was an independent positive predictor (p < 0.001). Female gender was an independent negative predictor of CCA intima-media thickness, whereas body mass index, ET-1 and C-reactive protein were independent positive predictors (p < 0.001). Conclusions: The results suggest several novel modifiable mechanisms related to the short-term effects of dialysis that are potentially implicated in the development of uremic cardiomyopathy.

Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey*e-mail: msenel@fatih.edu.tr; cnergiz@fatih.edu.trReceived: October 4, 2012Accepted: December 25, 2012Published online: April 2, 2013AbstractAn amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and sub-sequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is de-scribed. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction andoxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at+0.5 V with a high sensitivity (5.94 mA/mM) and relatively good response time (~12 s) in a wide concentrationrange of 1–17 mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.Keywords: Polyaniline, Glucose Oxidase, Biosensor, Boronic acidDOI: 10.1002/elan.201200535