U skripti "Osnove histologije i citologije" opisane su metode analiziranja tkiva i stanica, osnovne strukture stanica i njihove funkcije, epitelno tkivo, vezivno tkivo, masno tkivo, hrskavicno tkivo, kostano tkivo i zglobovi, misicno tkivo, živcano tkivo, žilni sustav, krvne stanice, imunosni sustav, probavni sustav, žljezde pridružene probavnoj cijevi, disni sustav, mokracni sustav, endokrini sustav, ženski i muski spolni sustav, koža i osjetni sustav.

Abstract The expression of 70 kDa protein zeta-associated protein (ZAP-70) in chronic lymphocytic leukemia (CLL) has been used to detect those patients with more aggressive disease. The aim of this study was to determine the proliferative activity of ZAP-70(+) leukemic cells by immunocytochemical methods. The study was undertaken on native blood marrow (BM) and peripheral blood (PB) smears from 65 patients with CLL. ZAP-70 was expressed in leukemic cells of 35 patients (54%). We demonstrated that ZAP-70 immunoreactivity correlated with Rai 0–IV (p = 0.002) and Binet A–C stages (p < 0.001), total tumor mass (TTM score) (p < 0.001), β2-microglobulin (p = 0.006), atypical lymphocytes (p < 0.001) and proliferative activity in bone marrow and peripheral blood (p = 0.014, p = 0.002, respectively) using χ2 test and Mann–Whitney test. ZAP-70 protein expression is in direct correlation with the poorer prognostic parameters, which additionally confirms the successful method of detection of ZAP-70 expression. Higher Ki-67 expression in BM and PB smears of patients with ZAP-70(+) disease indicates higher proliferating compartments, which may contribute to poorer prognosis.

Bone Marrow / Plasma Cell Neoplasms & Chronic Leukemic Lymphoproliferative Diseases - WORKSHOP....

A 61-year-old woman presented with an isolated, painless, slightly enlarged right laterocervical lymph node without any other signs and symptoms of disease. Laboratory test including hematological and biochemical parameters were normal. A cervical ultrasonography demonstrated one lymph node (10 mm) on the right laterocervical side and one small reactive lymph node on the left laterocervical side. The fine needle aspiration (FNA) smears revealed a polymorphic population of cells composed of lymphocytes, histiocytes, epitheloid cells, plasma cell, tingible body macrophages and macrophages infiltrated with Leishmania amastigotes. Treatment was initiated with Stiboglukonat Na (Pentostam) and led to a full recovery.