Background Serostudies are important resources when following pandemics and predicting their further spread, as well as determining the length of protection against reinfection and vaccine development. The aim of this study was to update data on the prevalence of seropositive individuals in Canton Sarajevo, Bosnia and Herzegovina (B&H) from September 2020 to May 2021. Methods Anti-SARS-CoV-2 antibodies were quantified using an electrochemiluminescence immunoassay. Results Compared to the period April–July 2020, when anti-SARS-CoV-2 antibodies were detected in 3.77% of samples, one year later (May 2021) the estimated percentage within the same population of the urban Canton Sarajevo was 29.9% (5,406/18,066). Of all anti-SARS-CoV-2 Ig-positive individuals, 53.27% were men, and 69.00% were of 50 years of age or younger. Also, the current update found the individuals 50 years of age or younger to be more frequently anti-SARS-CoV-2 Ig positive compared to older individuals. On the other hand, higher median anti-SARS-CoV-2 Ig levels were found in individuals > 50 years old than in younger individuals, as well as in men compared to women. Seropositivity gradually increased from September 2020 to May 2021, with the lowest frequency of positive cases (3.5%) observed in September 2020, and the highest frequency (77.7%) in January 2021. Conclusion Our results provided important seroprevalence data that could help in planning restrictive local public health measures to protect the population of Sarajevo Canton, especially considering that at the time of the study the vaccines were virtually inaccessible to the general population not belonging to any of the high-priority groups for vaccination.

The aim of this paper is to analyse the morphometric characteristics, length-weight relationship and condition factor of pumpkinseed sunfish (Lepomis gibbosus) from three reservoirs (Modrac, Vidara and Sniježnica) from the area of north-eastern Bosnia and Herzegovina (BiH). The total sample was 61 individuals. The following morphometric parameters were measured: total length, standard length, forked length, head length, mouth width, mouth height, first gill arch length, intestine length and body mass. Length-weight relationship was described by the equation: W=a TLb. Fulton’s condition factor (K) was determined using the equation: K=100 (W/L3). Statistical analysis was performed using the ANOVA to determine differences in morphometric characteristics of individuals between the sites (populations). L. gibbosus from all three populations showed positive allometric growth, and the highest value of condition factor was fish from the reservoir Vidara. In accordance with this result, most of the morphometric characteristics of individuals from the reservoir Vidara were significantly higher compared to individuals from Modrac and Sniježnica reservoirs. This result leads to the conclusion that the L. gibbosus adapted best in Vidara reservoir.

The odontogenic keratocyst (OKC) may occur at any age. However, it mostly occurs during the second and third decades of life. Compared to other odontogenic cysts, this type occurs with a frequency of 5-15%. It is more common in the mandible region and in the male sex. Histologically, odontogenic keratocysts are characterized by the presence of an external connective tissue capsule, with keratinizing lining of the epithelium consisting of 5-8 cell layers with marked palisadisation of polarized basal cells and a corrugated parakeratin layer. The objective of this study is to present cases of odontogenic keratocysts, with reference to the latest classification and dilemmas in therapeutic doctrine. This project was realized in the form of descriptive studies, specifically in a series of cases. A collection of four individual cases was found at the Department of Oral Surgery. Due to the proper approach towards diagnosis, adequate and detailed histopathological analysis, and suitable therapeutic procedures, all cases of odontogenic keratocysts were successfully treated without complications. Enucleation of OKC, with a regular follow-up, proved to be the effective therapeutic choice for the patients described in this paper. Only in the case of recurrence would we consider other therapeutic options, primarily enucleation in combination with Carnoy's solution.

BACKGROUND/AIMS The best treatment for an avulsed tooth is immediate replantation. If this is not possible, a proper transport medium is required for the maintenance of viability of the periodontal ligament cells (PDL). The aim was to systematically review the efficacy of different storage media used for the survival of PDL cells of avulsed teeth in the in vitro setting. METHODS The search strategy was based on the MeSH keywords in PubMed/MEDLINE: "Transport media for avulsed teeth," "Storage media for avulsed teeth," "Knocked out teeth," "Tooth avulsion," "Biological transport of avulsed tooth," "Cell survival of avulsed tooth," "Cell viability of avulsed tooth," "Tooth replantation," and "Periodontal ligament in avulsed teeth." The "AND" and "OR" Boolean operators were applied to combine keywords. Each study was evaluated for eight criteria, including use of human PDL, in vitro cell culture models, the number of passages, types of storage media, percentages of surviving PDL cells, pH and osmolality of storage media, and the type of test used to asses PDL viability. RESULTS In 15 selected studies, nine storage media (HBSS, tap water, DMEM, milk, saliva, 10% and 20% propolis, Gatorade, and Viaspan) were analyzed at six time points. For storage up to 2 hours, HBSS, DMEM, milk, 10% propolis, 20% propolis, and Viaspan conserved more than 80% of PDL viability. For storage at 24 hours, Viaspan showed best cell survival at 88.4%, followed by DMEM (70.9%) and 10% propolis (68.3%). Milk and HBSS showed similar PDL survival at 24 hours (57.2% and 57.3%, respectively). CONCLUSIONS Milk remains the most convenient, cheapest, and readily available solution in most situations while also being capable of keeping PDL cells alive. Further studies are required to evaluate the efficacy of more commonly found storage media besides milk.

Several association studies focusing on FTO gene polymorphisms have been published in the past years; however, the association between FTO -related conditions and FTO gene variants remains unexplained. Population genetics and association studies of different populations provide a valuable tool for further research. Thus, the aim of this systematic review is to summarize current knowledge on the FTO SNP rs9939609, and its association with presumably related conditions. The study included original research articles collected from PubMed and ResearchGate databases that were published in the period between 2007 and November 2017, and that provide information on rs9939609 mutant allele frequency and its probable association with any condition suspected of being related to the mutant allele. Genotype data was extracted and analyzed, and missing data was obtained from secondary sources. Short summaries of relevant studies from primary sources are organized in an overview table. The results of the systematic review suggest that mutant allele A is the most prevalent in European populations and least frequent on the Far East. In addition, it has been concluded that allele A is a good tool for the prediction of an increased risk of higher-than-normal BMI in a person carrying it, as well as that allele A should be further analyzed as a possible risk marker for type 2 diabetes mellitus and polycystic ovary syndrome development.

This paper presents a review of different storage media and their efficiency in retaining periodontal ligament (PDL) cell vitality before replantation. After avulsion of a tooth, immediate replantation is recommended, however this is often not possible. Therefore, storage media that are affordable and readily available are required to avoid resorption and tooth death by preservation of the PDL cells. Numerous studies have been undergone and use of media such as Hank’s Balanced Salt Solution (HBSS), milk, aloevera, coconut water, and et cetera have been trialed. In order to find the storage media that best meets the price/quality ratio, this study compares the length of time that the aforementioned solutions for retaining PDL vitality, their pH and osmolality, and discusses their efficiency in terms of other in vitro experiments. The media that are compared in this study are Hank’s Balanced Salt Solution (HBSS), milk, aloevera, coconut water, egg albumin, and contact lens solution. Results provide that HBSS and coconut water have the longest retention time, however milk, aloevera and egg albumin are also effective for shorter periods of time. Keywords— Periodontal ligament cells; avulsion; resorption; storage media; vitality

This paper presents a system for prediction of naphthalene concentration in the liver and lung compartments of a micro cell culture analog apparatus (μCCA) based on the Artificial Neural Network (ANN). The implemented ANN can be used to simulate organ and circulatory system reactions in terms of residual naphthalene concentrations. For neural network training, 100 samples were used and additional 100 samples were used for subsequent validation. For the lung compartment, the accuracy of prediction of naphthalene concentration is 97% and the accuracy of concentration prediction in liver compartment is 95%. Implemented neural network for prediction of residual concentration of naphthalene in lung and liver compartments of a μCCA uses the fraction of exiting stream that re-enters the microcircuit as input value. This system can be used for simulating the organ and circulatory system reactions before conducting experiments on micro cell culture analog apparatus.

INTRODUCTION Growth and development of children can be observed on individual and population level. Anthropometric measures are main indicators of that development. Secular trend of anthropometric measures increase in BiH and in the world is most often observed during the adolescence period. Changes in anthropometric measures are also investigated in newborns (body length, birth weight, head circumference, upper arm circumference and others) and are correlated with different endogenous and exogenous factors. Purpose of this paper is to analyze birth weight, body length and head circumference in newborns from Gracanica area, which were born in the period from 2007 to 2008, compare them with information's collected in 1998 and 1999, and then determine eventual secular trend. EXAMINEES AND METHODS Examinees were babies born in period from January 1st to December 31st 1998, and from January 1st 2007 to December 28th 2008. The method was retrospective and used information is from The Book of Protocols at the Department of Gynecology and Obstetrics, Dr M. Beganović Hospital in Gracanica. In observed period (1998 and 1999, 2007 and 2008) more girls was born than boys (N = 1801). RESULTS Newborns in Gracanica (2007-2008) weighted 3564 grams and female newborns weighted 3321+/- 333 grams in total sample. Body length in female newborns is 55.37; and male newborns 56.32. CONCLUSIONS Acceleration of growth of observed anthropometric parameters was established in newborns from Gracanica, in the period from 1998 to 2008 (for birth weight it is 84.96 g/dec; for body length it is 1,13 cm/dec and for head circumference it is 0.34 cm/dec).