SUMMARY CONFLICT OF INTEREST: none declared. Introduction Brucella endocarditis (BE) is a rare but severe and potentially lethal manifestation of brucellosis. Pre-existing valves lesions and prosthetic valves (PV) are favorable for BE. Case report We represent the case of a 46-year-old man who was treated at the Clinic for Infectious Diseases, Clinical Center of Sarajevo University, as blood culture positive (Brucella melitensis) mitral and aortic PV endocarditis. He was treated with combined anti-brucella and cardiac therapy. Surgical intervention was postponed due to cardiac instability. Four months later he passed away. Surgery was not performed.

INTRODUCTION Osteoarticular manifestations of human brucellosis occur in 20-40% of patients while spondylodiscitis is the most severe form of the bone and joint structures involvements. AIM The aim of this paper is tho show clinical and radiological caracteristics of osteoarticular forms of brucellosis, with special reference to spondylodiscitis. MATERIAL AND METHODS The medical histories of 120 hospitalized patients at the Clinic for Infectious Diseases, Clinical Center of Sarajevo University, diagnosed with brucellosis, were analyzed. RESULTS Osteoarticular manifestations had sixty-nine patients, representing 78.4% of all localized forms of the disease. Spondylodiscitis represents 40.6% of all osteoarticular manifestations of the disease. Nine patients (32.1%) had paravertebral and paraspinal abscess. Median diagnostic interval for spondylodiscitis (116 +/- 160 days) was almost twice prolonged compared to the arthritis and sacroilitis (p < 0.05). The most common radiological manifestations were erosions of the vertebral surface (67.8%). Computerized tomography confirmed inflammation in 85.2% of the patients, while magnetic resonance imaging (MRI) showed radiological alterations in all patients (100%). DISCUSSION AND CONCLUSION Osteoarticular manifestations are the most common localised forms of brucellosis. The frequency of spondylodiscitis is in relation to duration of the diagnostic time. MRI shows a high degree of sensitivity to inflammatory changes of spine and "Pedro Pons' sign" is patognomic radiological alteration.

During the last several years, brucellosis has become an important public-health problem on a large territory part of Bosnia and Herzegovina. The disease belongs to the zoonosis group, and can be caused by several bacterium species from Brucella genus. For human and veterinarian medicine, B. abortus, B. melitensis, B. suis and B. canis from Brucella genus are important, while other brucella species are found only in animals. The results of laboratory process of isolating Brucella melitensis, as well as of detection of specific antibacterial antibodies, are presented in this work. Namely, B. melitensis was isolated from blood samples (chemo-culture), as a causal agent of disease in one sixty years-old patient, treated during 2001. In pair serum samples of the patient, the presence of specific anti-brucella antibodies was confirmed qualitatively and quantitatively. In the serum I, ELISA test confirmed the presence of specific IgM antibodies of 25,7 U/ml, and IgG antibodies of 252 U/ml. In the serum II, IgM antibodies of 24,9 U/ml, and IgG antibodies of 311 U/ml were found. These results suggest and confirm established work diagnosis, and etiology causality of the disease with isolated bacterium.

a large territory part of Bosnia and Herzegovina. The disease belongs to the zoonosis group, and can be caused by several bacterium species from Brucella genus. For human and veteri- narian medicine, B. abortus, B. melitensis, B. suis and B. canis from Brucella genus are impor- tant, while other brucella species are found only in animals. The results of laboratory process of isolating Brucella melitensis, as well as of detection of specific antibacterial antibodies, are presented in this work. Namely, B. melitensis was isolated from blood samples (chemo-cul- ture), as a causal agent of disease in one sixty years-old patient, treated during . In pair serum samples of the patient, the presence of specific anti-brucella antibodies was confirmed qualitatively and quantitatively. In the serum I, ELISA test confirmed the presence of specific IgM antibodies of , U/ml, and IgG antibodies of  U/ml. In the serum II, IgM antibodies of , U/ml, and IgG antibodies of  U/ml were found. These results suggest and confirm established work diagnosis, and etiology causality of the disease with isolated bacterium.

During the period from April 1992 to April 1993 at the Clinic for infectious Disease in Sarajevo were hospitalized 213 patients. The major causative agents were different kinds of Shigella. Shigella sonnei with 159 (74.6%) was the most frequent isolated organism, then subsequent Shigella species with 38 (23.9%) and Shigella flexneri 3 with 23 (14.5%). Bacillary dysentery (Shigellosis) was manifested as acute gastroenteritis with 60 patients (37.7%). This disease was manifested in the form of acute enterocolitis in the same number and there were also 39 cases or 24.5% of enterocolitis acuta haemorrhagica. The patients were treated with symptomatic or dietary regimen in 69 (43.4%). As a specific therapy 36 (22.6%) patients were received Trimethoprim-sulfamethoxazole, 28 (17.6%) Chloramphenicol and 24 (15.1%) Pefloxacin, 56 patients were treated with Nifuroxazid (Ercefuryl) only. The most sensitivity of isolated organism were shown up against Pefloxacin (100%), aminoglycosides (99.4%), Chloramphenicol (96.9%) and Cephalosporins (60.4%). The lowest sensitivity, at the same rate had Ampicillin and Trimethoprim sulfamethoxazole. All patients were recovered, there were no complications. The diseases were manifested as acute gastroenterocolitis (28 or 13.1%), and acute enterocolitis (17 or 8.0%) at the patients with bacteriological unknown causative agents. Salmonella was recorded at 9 patients.