8
7. 4. 2007.
Isolation of Mycoplasma bovis from the respiratory tract of cattle in Bosnia and Herzegovina
a 1911 base pair gene fragment. The thermal profile for the reaction included an initial denaturation step at 94°C for 12 minutes, followed by 35 cycles consisting of a denaturation step at 94°C for 30 seconds, annealing at 57°C for 1 minute and extension at 72°C for 1·5 minutes, with a final extension step at 72°C for 10 minutes. The