ARRIVAL OF CARBAPENEMHYDROLYZING- OXACILLINASES IN ACINETOBACTER BAUMANNII IN BOSNIA AND HERZEGOVINA
Multidrug-resistant Acinetobacter baumannii (MDR-AB) is an important threat for critically ill patients. It can infect the respiratory tract, blood, soft tissues, urinary tract and central nervous system. Recently, carbapenem-resistance was observed in A. baumannii clinical isolates from Bosnia and Herzegovina. This prompted us to analyze these isolates with regards to genotypic diversity, antibiotic susceptibility and occurrence of acquired carbapenem resistance genes. Twelve carbapenem-resistant isolates were collected at a University hospital during two different periods of 2011 and 2015-2016: four isolates in 2011 and eight isolates 2015-2016 and compared to determine the dynamic changes in carbapenemase resistance mechanisms and population structure. All twelve isolates were positive for intrinsic bla OXA-51-like , nine for bla OXA-40-like and one for the bla OXA-23-like gene. IS Aba1 was found upstream of bla OXA-51 in all and upstream of bla OXA-23-like gene in one isolate. Sequencing of the selected PCR products revealed the presence of OXA-72 β-lactamase (strain 1) and OXA-23 β-lactamase (strain 41). WGS of the selected isolate (AB 5) revealed the presence of bla OXA-72 , chromosomal genes bla OXA-69 and bla ADC . Moreover, the aac (3 )-1a and aadA1 genes encoding aminoglycoside resistance, and sul1 encoding sulphonamide resistance were identified. PFGE and rep-PCR revealed two clones containing highly similar isolates positive for OXA-40-like; one from 2011 and the other from 2015-2016.