Development of Amperometric Glucose Biosensor Based on Reconstitution of Glucose Oxidase on Polymeric 3-Aminophenyl Boronic Acid Monolayer
Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey*e-mail: msenel@fatih.edu.tr; cnergiz@fatih.edu.trReceived: October 4, 2012Accepted: December 25, 2012Published online: April 2, 2013AbstractAn amperometric biosensor for determining glucose based on deflavination of the enzyme glucose oxidase and sub-sequent reconstitution of the apo-protein with a complexed flavin adenine dinucleotide (FAD) monolayer is de-scribed. The GOx-reconstituted electrode exhibited excellent electrocatalytic activities towards the reduction andoxidation of hydrogen peroxide as well. The prepared biosensor showed an excellent performance for glucose at+0.5 V with a high sensitivity (5.94 mA/mM) and relatively good response time (~12 s) in a wide concentrationrange of 1–17 mM (correlation coefficient of 0.9998). The applicability to blood analysis was also evaluated.Keywords: Polyaniline, Glucose Oxidase, Biosensor, Boronic acidDOI: 10.1002/elan.201200535