Pathophysiological Effect of Oxygen Free Radicals on Mobility and DNA Integrity of Human Seminal Spermatozoa
Production of free oxygen radicals in greater volume than it is possible for human organism to neutralize, can affect quality of sperm. Reactive oxygen species (ROS) have an effect on mobility of human spermatozoids by changing the structure of membrane and the function of mitochondria, where they are primarily generated. This can affect DNA integrity of spermatozoids and compromise their vitality. Seven male patients, in age between 27 and 44, were examined by standard semen analysis, nitroblue tetrazolium (NBT) assay and sperm vitality test based on eosin and nigrosin staining. From patients with positive NBT test, 40% of the patients had normal spermiogram results, while 60% of them had decreased sperm concentration, motility, or both of these parameters below the referent value. In 20% cases the output of eosin-nigrosin test was below 58%. The NBT test and eoisn-nigrosin test are simple, effective and available tests which can help in better diagnosis of male infertility and should be integrated in routine