Effects of a modified clinoptilolite zeolite on growth performance, health status and detoxification of aflatoxin B1 and ochratoxin A in male broiler chickens

ABSTRACT 1. The aim of the present study was to test the ability of an in-feed modified clinoptilolite zeolite-based mycotoxin binding substance (Minazel® Plus, Patent Co, Misicevo, Serbia; MP) to prevent gastrointestinal absorption of aflatoxin B1 (AFB1) and ochratoxin A (OTA) and its effects on health status and performance parameters of broilers. 2. A total of 375, 1 d old male broiler chickens (Cobb 500) were used for a total trial period of 42 d (from hatch to 42 d of age). Animals were randomly allocated to five treatment groups (T1-T5), in 25 pens (15 male broilers per pen, five pens per treatment). T1 was the control maize-based diet without the addition of mycotoxins, or the test product. T2 and T3 groups received contaminated maize in the diet containing 0.02 mg AFB1/kg feed and 0.1 mg OTA/kg feed, whereas T4 and T5 groups received 0.05 mg AFB1/kg feed and 0.5 mg OTA/kg feed. The MP was added to T3 (1 g/kg feed), and T5 (2 g/kg feed) groups. 3. Results showed that exposure to AFB1 and OTA at low or moderate levels, as used in this study, did not markedly affect growth performance, blood profile or organ weights. Improvements in feed conversion ratio (FCR) were observed in birds receiving MP, whereby FCR of T3 group was improved in comparison with T2 group, although there was no significant difference between T5 and T4 groups. However, average body weight gain (ABWG) was improved in the T5 group compared to T4, but not in the T3 versus T2 group comparison. 4. For serum biochemical parameters, glutamate-dehydrogenase (GLDH) was significantly improved in T5 birds in comparison with T4. The addition of MP significantly decreased residue levels of AFB1 in liver and OTA in the spleen of the treated groups. 5. The improvements in productive performance and reduction of mycotoxin residue levels in tissues demonstrated a beneficial effect of MP in cases of concurrent AFB1 and OTA ingestion by broilers.