Eff ect of met-enkephalin on chromosomal aberrations in the lymphocytes of the peripheral blood of patients with multiple sclerosis

Endogenious opiod met-enkephalin throughout previous research manifested cytoprotective and anti-infl ammatory eff ects. Previous research suggests that met-enkephalin has cytogenetic eff ects. Reducement in the frequency of structural chromosome aberrations as well as a suppressive eff ect on lymphocyte cell cycle is found. It also reduces apoptosis in the blood samples of the patients with immune-mediated diseases. Met-enkephalin exerts immunomodulatory properties and induces stabilization of the clinical condition in patients with multiple Sclerosis (MS). Th e goal of the present research was to evaluate met-enkephalin in vitro eff ects on the number and type of chromosome aberrations in the peripheral blood lymphocytes of patients with MS. Our research detected disappearance of ring chromosomes and chromosome fragmentations in the cultures of the peripheral blood lymphocytes treated with met-enkephalin (. μg/mL). However, this research did not detect any signifi cant eff ects of met-enkephalin on the reduction of structural chromosome aberrations and disappearance of dicentric chromosomes. Chromosomes with the greatest percent of inclusion in chromosome aberrations were noted as: chromosome , chromosome  and chromosome . Additionally, we confi rmed chromosome  as the most frequently included in translocations. Furthermore, met-enkephalin eff ects on the increase of the numerical aberrations in both concentrations applied were detected. Th ose fi ndings should be interpreted cautiously and more research in this fi eld should be conducted. ©  Association of Basic Medical Sciences of FB&H. All rights reserved