Synthesis, structure and antibacterial activity of 3-substituted derivatives of 4-hydroxycoumarin
Background and purpose: Having in mind the results of our previous work, which suggested antimicrobial activity of some 3-cynnamoyl-4-hydroxycoumarins, we carried out the synthesis of several new derivatives of this group. The microbiological activity of compounds was tested by the diffusion and dilution methods on various species of bacteria. The aim of the study was to evaluate the influence of the substituents on antimicrobial activity. Material and methods: A series of new 3-cynnamoyl-4-hydroxycoumarins was prepared by the reaction of nucleophylic addition from 3-acetyl-4-hydroxycoumarin acting on appropriate aromatic aldehydes. The microbiological activity of compounds was tested by the diffusion and dilution methods on species of bacteria Pseudomonas aeruginosa, Echerichia coli, Salmonella typhimurium, Bordatella bronchiseptica, Bacillus subtilis, and Staphyloccocus aureus. Results: The synthesis of new 3-cynnamoyl-4-hydroxycoumarins was carried out. The elementary content of the synthesized compounds was confirmed by elementary analysis, and structures were confirmed with IR-spectrophotometry and 1 H-NMR spectrophotometry.