Analysis of Some Leguminous Plants For Pyrimidine Constituents
The pyrimidine and pyrimidine-derived secondary compounds of Phaseolus aureus and Glycine max were investigated. Batches, each consisting of one hundred 12-day-old seedlings of either P. aureus or of G. max were separately extracted in cold dilute (0.6 M) perchloric acid. After preliminary purification of extracts by charcoal adsorption and elution, and by PVP treatment, the extracts were subjected to paper chromatography and high voltage paper electrophoresis. The UV- absorbing bands were detected by viewing chromatograms and electrophoretograms under UV-light and, where possible, identified. The results presented in this work show that these experimental plants do not synthesize and accumulate any significant amount of a pyrimidine-derived secondary product.