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15. 7. 2025.
In-depth patient-specific analysis of tumor heterogeneity in melanoma brain metastasis: Insights from spatial transcriptomics and multi-region bulk sequencing
Highlights • MBM tumors show significant intertumor and intratumor heterogeneity in cellular composition, gene mutations, and pathway enrichment.• Therapy-treated tumors (P2, P4) exhibited immune activation, while untreated tumors (P1, P3) showed cold tumor signatures.• P1 and P4 tumors were enriched in CAFs, correlating with epithelial-mesenchymal transition and angiogenesis pathways.• Proteomic analysis revealed activation of oncogenic pathways like JAK-STAT, NF-κB, MAPK, and EMT, driving tumor progression.