DIRECT IMMUNOFLUORE- SCENCE AND IMMUNO- HISTOCHEMISTRY IN DIAGNOSTICS OF GLOMERULONEPHRITIS
Th e needle biopsies from transplanted and native kidneys have been processed and a pro- spective analysis of pattern, intensity and distribution of immunoglobulin deposits (IgA, IgG and IgM) and complement components (Cc and Cq) identifi ed in these lesions has been carried out by immunohistochemistry with three step immunoperoxidase, in the period from to . Th ose deposits were previously detected and analyzed by immunofl uorescence. Th e samples consisted of renal biopsies, previously diagnosed with glomerulonephritis and positive immunofl uorescence and renal biopsies without morphologic changes and deposits on immunofl uorescence. , of the analyzed samples showed the identical results of the deposits of immunoglobulin and components of the complement with both, immuno- histochemistry and immunofl uorescence method. Sensitivity of the immunohistochemistry method with three step immunoperoxidase for all analyzed immunoglobulin and complement components is high (,), while specifi city for the same method is ,. Standardized method of the three step immunoperoxidase on the paraffi n embedded, formalin fi xed needle renal biopsies could successfully replace the immunofl uorescence method in diagnostic of GN, with the emphasis on a follow up and control of each single step in the procedure of the method.